Green Synthesis of AgNPs and Fe2O3NPs Using Garlic Plant and Study their Anticancer Activity against Cervical Cancer Cells
Main Article Content
Abstract
In this study, silver and iron nanoparticles were biosynthesized using the garlic plant, an economical and environmentally friendly method. A blend of chitosan (CS) and polyvinylalcohol (PVA) was prepared. The silver nanoparticles (AgNPs), iron oxide nanoparticles (Fe2O3NPs), and the (CS/PVA) blend were characterized by X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), and Field Emission Scanning Electron Microscopy (FE-SEM) analyses. The FE-SEM images revealed that Fe2O3NPs were cubical and hexagonal, and AgNPs were spherical aggregates. AgNPs with blend (CS/PVA) and Fe2O3NPs with blend (CS/PVA) and (Fe2O3NPs/blend (CS/PVA) /AgNPs) composite were synthesized and tested for anticancer activity against cervical cancer cells (Hela) using the MTT assay. Best kills and the highest inhibitory effect were observed in (AgNPs/blend (CS/PVA)) and (Fe2O3NPs/Blend (CS/PVA)/AgNPs) composite. These findings demonstrated the method's ability to synthesize nanocomposites with desirable physical, chemical and biological properties. Therefore, these findings demonstrate the new antibacterial and robust cytotoxicity features of the nanocomposite material, which has promising medical applications.
Article Details
Issue
Section
This work is licensed under a Creative Commons Attribution 4.0 International License.
© 2023 The Author(s). Published by College of Science, University of Baghdad. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License.
How to Cite
References
H. F. Al-Rubai, A. K. H. Al-Dahan, and B. A. Jasim, Iraqi J. Sci. 64, 1592 (2023).
H. H. Abbas and S. N. Mazhir, Iraqi J. Phys. 21, 9 (2023).
M. J. Mitchell, M. M. Billingsley, R. M. Haley, M. E. Wechsler, N. A. Peppas, and R. Langer, Nat. Rev. Drug Discov. 20, 101 (2021).
A. Bokare, A. Takami, J. H. Kim, A. Dong, A. Chen, R. Valerio, S. Gunn, and F. Erogbogbo, ACS Omega 4, 4650 (2019).
E. a. A. Kareem, A. E. Sultan, and H. M. Oraibi, Ibn AL-Haitham J. Pur. Appl. Sci. 36, 177 (2023).
K. Cho, X. Wang, S. Nie, Z. Chen, and D. M. Shin, Clin. Canc. Res. 14, 1310 (2008).
M. S. Ricci and W.-X. Zong, The Oncologist 11, 342 (2006).
K. H. Bae, H. J. Chung, and T. G. Park, Molec. Cells. 31, 295 (2011).
J. Wang, D. Mongayt, and V. P. Torchilin, J. Drug Targ. 13, 73 (2005).
K. Y. Choi, H. Chung, K. H. Min, H. Y. Yoon, K. Kim, J. H. Park, I. C. Kwon, and S. Y. Jeong, Biomaterials 31, 106 (2010).
E. Blanco, A. Hsiao, A. P. Mann, M. G. Landry, F. Meric‐Bernstam, and M. Ferrari, Cancer Sci. 102, 1247 (2011).
O. C. Farokhzad, J. Cheng, B. A. Teply, I. Sherifi, S. Jon, P. W. Kantoff, J. P. Richie, and R. Langer, Proce. Nat, Acad. Sci. 103, 6315 (2006).
A. N. Lukyanov, T. A. Elbayoumi, A. R. Chakilam, and V. P. Torchilin, J. Cont. Rel. 100, 135 (2004).
S. S. Aleksenko, A. Y. Shmykov, S. Oszwałdowski, and A. R. Timerbaev, Metallomics 4, 1141 (2012).
J. D. Byrne, T. Betancourt, and L. Brannon-Peppas, Adv. Drug Deliv. Rev. 60, 1615 (2008).
V. P. Torchilin, Advan. Drug Deliv. Rev. 58, 1532 (2006).
H. Wang, T. Ding, J. Guan, X. Liu, J. Wang, P. Jin, S. Hou, W. Lu, J. Qian, and W. Wang, ACS Nano 14, 14779 (2020).
K. H. Lee, G. Yang, B. E. Wyslouzil, and J. O. Winter, ACS Appl. Poly. Mat. 1, 691 (2019).
Y. Liu, G. Yang, D. Zou, Y. Hui, K. Nigam, A. P. Middelberg, and C.-X. Zhao, Indus. Eng. Chem. Res. 59, 4134 (2019).
N. Pinkerton, L. Behar, K. Hadri, B. Amouroux, C. Mingotaud, D. Talham, S. Chassaing, and J.-D. Marty, Nanoscale 9, 1403 (2017).
X. Cui, J. Wang, X. Zhang, Q. Wang, M. Song, and J. Chai, Langmuir 35, 9255 (2019).
S. Maiz-Fernández, L. Pérez-Álvarez, L. Ruiz-Rubio, R. Pérez González, V. Sáez-Martínez, J. Ruiz Pérez, and J. L. Vilas-Vilela, Polymers 11, 742 (2019).
S. Y. Lee, E.-H. Hong, J. Y. Jeong, J. Cho, J.-H. Seo, H.-J. Ko, and H.-J. Cho, Biomat. Sci. 7, 4624 (2019).
R. Wang, Y. Luo, S. Yang, J. Lin, D. Gao, Y. Zhao, J. Liu, X. Shi, and X. Wang, Sci. Rep. 6, 33844 (2016).
J. Zhao, Z. Wan, C. Zhou, Q. Yang, J. Dong, X. Song, and T. Gong, Pharmac. Res. 35, 1 (2018).
W. Wei, J. Sun, X.-Y. Guo, X. Chen, R. Wang, C. Qiu, H.-T. Zhang, W.-H. Pang, J.-C. Wang, and Q. Zhang, ACS Appl. Mat. Inter. 12, 14839 (2020).
H. Patil, X. Feng, X. Ye, S. Majumdar, and M. A. Repka, AAPS J. 17, 194 (2015).
D. Chen, K. T. Love, Y. Chen, A. A. Eltoukhy, C. Kastrup, G. Sahay, A. Jeon, Y. Dong, K. A. Whitehead, and D. G. Anderson, J. Amer. Chem. Soci. 134, 6948 (2012).
R. Ciriminna, A. Fidalgo, V. Pandarus, F. Beland, L. M. Ilharco, and M. Pagliaro, Chem. Rev. 113, 6592 (2013).
D. Desai, Y. A. Guerrero, V. Balachandran, A. Morton, L. Lyon, B. Larkin, and D. E. Solomon, Nanomedicine : nanotechnology, biology, and medicine 35, 102402 (2021).
K. Zhai, X. Pei, C. Wang, Y. Deng, Y. Tan, Y. Bai, B. Zhang, K. Xu, and P. Wang, Int. J. Bio. Macromolec. 131, 1032 (2019).
A. Fabozzi, F. Della Sala, M. Di Gennaro, N. Solimando, M. Pagliuca, and A. Borzacchiello, Poly. Chem. 12, 6667 (2021).
T. Baby, Y. Liu, A. P. Middelberg, and C.-X. Zhao, Chem. Eng. Sci. 169, 128 (2017).
J. Ma and C.-W. Li, Sens. Actuat. B Chem. 262, 236 (2018).
D. R. Reyes, H. Van Heeren, S. Guha, L. Herbertson, A. P. Tzannis, J. Ducrée, H. Bissig, and H. Becker, Lab on a Chip 21, 9 (2021).
M. Arbyn, E. Weiderpass, L. Bruni, S. De Sanjosé, M. Saraiya, J. Ferlay, and F. Bray, Lan. Glo. Heal. 8, e191 (2020).
G. Bogani, F. Sopracordevole, V. Di Donato, A. Ciavattini, A. Ghelardi, S. Lopez, T. Simoncini, F. Plotti, J. Casarin, and M. Serati, Gyn. Oncol. 161, 173 (2021).
M. Scioscia, M. Noventa, S. Palomba, and A. Laganà, BJOG: Int. J. Obst. Gyn. 128, 2213 (2021).
C. Uwins, H. Patel, G. P. Bhandoria, S. Butler-Manuel, A. Tailor, P. Ellis, and J. Chatterjee, Clinic Onco. 33, e372 (2021).
D. Cibula, R. Pötter, F. Planchamp, E. Avall-Lundqvist, D. Fischerova, C. Haie-Meder, C. Köhler, F. Landoni, S. Lax, and J. C. Lindegaard, Virch. Archiv 472, 919 (2018).
V. Wagner, A. Dullaart, A.-K. Bock, and A. Zweck, Nat. Biotech. 24, 1211 (2006).
A. J. Haider, A. L. Abed, and D. S. Ahmed, Iraqi J. Sci. 57, 1203 (2016).
S. Sabeha and Z. J. Shanan, Ibn AL-Haitham J. Pur. Appl. Sci. 36, 91 (2023).
G. M. Saleh, Iraqi J. Sci. 61, 1289 (2020).
D. R. Ibraheem, N. N. Hussein, and G. M. Sulaiman, Iraqi J. Sci. 64, 2223 (2023).
S. M. Abdullah, A. a. S. Al-Hamdani, and F. A. Resheed, Ibn AL-Haitham J. Pur. Appl. Sci. 36, 201 (2023).
J. M. M. Mohamed, A. Alqahtani, T. V. A. Kumar, A. A. Fatease, T. Alqahtani, V. Krishnaraju, F. Ahmad, F. Menaa, A. Alamri, and R. Muthumani, Molecules 27, 110 (2021).
S. N. Mizil, E. A. Elkaaby, and M. Z. Al-Shammary, bn AL-Haitham J. Pur. Appl. Sci. 36, 60 (2023).
M. S. Samuel, S. Datta, N. Chandrasekar, R. Balaji, E. Selvarajan, and S. Vuppala, Nanomaterials 11, 3290 (2021).
E. Ibrahim, M. Zhang, Y. Zhang, A. Hossain, W. Qiu, Y. Chen, Y. Wang, W. Wu, G. Sun, and B. Li, Nanomaterials 10, 219 (2020).